Corpus 1,285 assessed · 1,186 scored · 647 reproduced ≥75 · 174 flagged ·∅ 73.9/100
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SRR350021

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

79/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Illumina Genome Analyzer II short-read RNA-seq of Lucilia sericata (green bottle fly) with 17.9 million reads and perfect Q30 100% (Q20 100%) provides exceptional-quality transcriptomics for this medically important blow fly, enabling wound-colonization and immune-gene profiling. The 39.3% GC and flawless base accuracy enable definitive isoform discovery and sensitive detection of genes regulating maggot-mediated wound therapy and fly reproduction.

Data type / assay
bulk-RNA-seq
Organism
Lucilia sericata
Instrument
Illumina Genome Analyzer II
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
1 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 967851180 reported
total reads 17923170 reported
n content pct 0.07 measured
pct q20 bases 100 measured
pct q30 bases 100 measured
gc content pct 39.3 measured
mean read length 54 measured
mean base quality 30 measured
adapter content pct 0.13 measured
duplication rate pct 42.32 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 79/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 100 measured ×1 100%
mean base quality 30 measured ×0.6 33%
adapter content pct 0.13 measured ×0.4 100%
duplication rate pct 42.32 measured ×0.4 73%
QC cost 29 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0