SRR350961

ENA first seen 2012

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

78/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

EST-based sequencing from Drosophila melanogaster on an Illumina HiSeq 2000, yielding 48.2 million short reads at perfect Q30 quality (100%) across 9.6 billion bases. The minimal N-content (0.009%) and elevated GC composition (53.3%) support comprehensive transcriptome mapping in this dipteran model.

Data type / assay

bulk-RNA-seq

Organism

Drosophila melanogaster

Instrument

Illumina HiSeq 2000

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 9631974600 reported

total reads 48159873 reported

n content pct 0.009 measured

pct q20 bases 100 measured

pct q30 bases 100 measured

gc content pct 53.3 measured

mean read length 80 measured

mean base quality 30 measured

adapter content pct 0 measured

duplication rate pct 43.17 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 78/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 100 measured ×1 100%

mean base quality 30 measured ×0.6 33%

adapter content pct 0 measured ×0.4 100%

duplication rate pct 43.17 measured ×0.4 71%

QC cost 48 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0