Corpus 1,284 assessed · 1,185 scored · 647 reproduced ≥75 · 173 flagged ·∅ 73.9/100
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SRR3547485

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

58/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

HiSeq 2000 short-read RNA-seq of Apis mellifera (honeybee) with 45.3 million reads but elevated N-content (0.15%) and lower Q30 75.9% (Q20 84%) provides very deep-coverage transcriptomics with moderate base quality, suitable for expression quantification despite QC concerns. The 39.6% GC and high read depth enable identification of highly expressed genes, though base-calling accuracy issues warrant filtering for sensitive isoform-level work.

Data type / assay
bulk-RNA-seq
Organism
Apis mellifera
Instrument
Illumina HiSeq 2000
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
1 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 2262623700 reported
total reads 45252474 reported
n content pct 0.145 measured
pct q20 bases 84 measured
pct q30 bases 75.9 measured
gc content pct 39.6 measured
mean read length 50 measured
mean base quality 31 measured
adapter content pct 0 measured
duplication rate pct 14.03 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 58/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 75.9 measured ×1 30%
mean base quality 31 measured ×0.6 50%
adapter content pct 0 measured ×0.4 100%
duplication rate pct 14.03 measured ×0.4 100%
QC cost 39 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0