SRR3547489

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

68/100 · D

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Bulk RNA-seq from Apis mellifera on an Illumina HiSeq 2000, producing 95.6 million short reads with lower Q30 coverage (78.7%) across 4.8 billion bases. The elevated N-content (0.149%) is notable; despite quality issues, the substantial read depth and moderate GC composition (39.7%) may support analyses with aggressive filtering.

Data type / assay

bulk-RNA-seq

Organism

Apis mellifera

Instrument

Illumina HiSeq 2000

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 4777824650 reported

total reads 95556493 reported

n content pct 0.149 measured

pct q20 bases 86.3 measured

pct q30 bases 78.7 measured

gc content pct 39.7 measured

mean read length 50 measured

mean base quality 31.9 measured

adapter content pct 0 measured

duplication rate pct 15 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 68/100

The D grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 78.7 measured ×1 44%

mean base quality 31.9 measured ×0.6 65%

adapter content pct 0 measured ×0.4 100%

duplication rate pct 15 measured ×0.4 100%

QC cost 47 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0