Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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SRR3651659

ENA first seen 2018

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

83/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:opus

Bulk small-RNA (miRNA-Seq) data from mouse on the Illumina HiSeq 2000, scoring 83/100 (grade B), meaning it is broadly reusable but with one notable caveat. The grade is held up by excellent base-call quality — 92% of bases at Q30 and a mean base quality of 34.7, with adapters fully trimmed (0% adapter content) — so per-base accuracy and read cleanliness are not concerns for downstream miRNA quantification. The one metric dragging the score down is the 87.9% duplication rate, which scored 0; in general RNA-seq that flags a library-complexity problem, though in miRNA-Seq high duplication is partly expected because mature miRNAs are short, few, and highly abundant, so judge it against small-RNA norms rather than treating it as outright failure. All the quality metrics here are directly measured (the duplication, Q30, adapter, and base-quality figures are real, not extrapolated), so this reading is firm rather than provisional — the main thing to confirm before reuse is whether the duplication reflects true biological abundance or PCR over-amplification.

Data type / assay
bulk-RNA-seq
Organism
Mus musculus
Instrument
Illumina HiSeq 2000
Platform
ILLUMINA
N numbers (samples, groups)
1 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 525539900 reported
total reads 10510798 reported
n content pct 0.035 measured
pct q20 bases 94.8 measured
pct q30 bases 92 measured
gc content pct 44.6 measured
mean read length 50 measured
mean base quality 34.7 measured
adapter content pct 0 measured
duplication rate pct 87.9 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 83/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 92 measured ×1 100%
mean base quality 34.7 measured ×0.6 100%
adapter content pct 0 measured ×0.4 100%
duplication rate pct 87.9 measured ×0.4 0%
QC cost 17 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0

Scientific quality

Based on hands-on reproduction of the papers that use this dataset. A reproducible paper that stands on this data is positive evidence; a flagged one is a prompt to look closer — never a verdict on the dataset itself without the evidence.

1 studies use it 1 reproduced mean score 89