SRR3652245

Bulk small-RNA (miRNA-Seq) data from mouse on the Illumina HiSeq 2000, this dataset earns a solid B (83/100) on the strength of its base-level quality: 92.5% of bases at Q30 and a mean base quality of 34.8 indicate clean, reliably called reads, and adapter content reading 0% means the libraries are well-trimmed and ready for alignment. The single metric dragging the grade down is an 86.59% duplication rate, which scored 0 and is the main reason this is a B rather than an A — though for a miRNA-Seq library this is partly expected, since a small, highly expressed pool of mature miRNAs naturally produces many identical reads, so treat it as a flag to watch rather than outright contamination. Because the evidence_strength is low (1) and the core size metrics (total_reads, total_bases) are reported rather than independently measured, this reading should be considered provisional and confirmed by a deeper measured QC pass before high-stakes reuse. On balance the data are trustworthy for differential miRNA expression, but verify that your downstream tool handles duplicates appropriately (e.g. UMI-aware or expression-based counting) given the high duplication.