SRR3652859

Bulk small-RNA (miRNA-Seq) data from mouse on an Illumina HiSeq 2000, and overall it's a solid, fully measured library that earns its B: base quality is excellent (Q30 at 89.4% and mean base quality ~33.9, both scoring 97–98/100), adapter contamination is effectively zero, and the QC reading is well-grounded since every quality metric here was actually measured rather than extrapolated. The one factor pulling the grade down is the 86% duplication rate, which scored 0/100 — though for miRNA-Seq this is expected and largely benign, because the small, highly expressed miRNA repertoire is naturally sequenced to redundancy, so much of that "duplication" reflects genuine biological abundance rather than PCR artifact. The main practical caveat is depth: at ~6M reads of 50 bp single-end data, the library is adequate for profiling abundant miRNAs but offers limited sensitivity for low-expression species, so reuse is well-suited to differential-expression of common miRNAs and less so to discovery of rare ones. Treat the quality numbers as reliable rather than provisional, but interpret the duplication metric in the small-RNA context before letting it deter reuse.