Corpus 1,273 assessed · 1,174 scored · 643 reproduced ≥75 · 169 flagged ·∅ 74.1/100
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SRR372650

ENA first seen 2018

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

83/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:opus

This is a bulk human RNA-seq run from an older Illumina Genome Analyzer IIx, and it earns a solid B (83/100) backed by full measured evidence (evidence_strength=1), so the reading is firm rather than provisional. The grade was held back almost entirely by mean_base_quality, which sits at Phred ~30 and scored only 33/100 under the heaviest weight — a Q30 average is actually respectable for this instrument generation, but it leaves less quality headroom than modern platforms, so expect somewhat reduced confidence in low-frequency variant or allele-specific calls. On the positive side, duplication is moderate at 31.8% (scored 96/100, typical and acceptable for RNA-seq library complexity) and adapter content is effectively zero, meaning reads are clean and need little trimming before alignment. Overall this is trustworthy, reusable expression data with no measured red flags; just treat the base-quality ceiling as the main limit if you intend to push it toward sensitive variant-level analysis.

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Instrument
Illumina Genome Analyzer IIx
Platform
ILLUMINA
N numbers (samples, groups)
1 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 4519862250 reported
total reads 53174850 reported
n content pct 26.194 measured
pct q20 bases 100 measured
pct q30 bases 100 measured
gc content pct 29.6 measured
mean read length 85 measured
mean base quality 30 measured
adapter content pct 0 measured
duplication rate pct 31.84 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 83/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 100 measured ×1 100%
mean base quality 30 measured ×0.6 33%
adapter content pct 0 measured ×0.4 100%
duplication rate pct 31.84 measured ×0.4 96%
QC cost 15 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0

Scientific quality

Based on hands-on reproduction of the papers that use this dataset. A reproducible paper that stands on this data is positive evidence; a flagged one is a prompt to look closer — never a verdict on the dataset itself without the evidence.

1 studies use it 1 reproduced mean score 89