SRR3948542

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

91/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Short-read bulk RNA-seq from Megalopta genalis on an Illumina HiSeq 2500 with 13.1 million reads spanning 2.6 billion bases and strong Q30 coverage (95%). The minimal N-content (0.004%) and moderate GC composition (43.6%) enable expression profiling in this nocturnal bee.

Data type / assay

bulk-RNA-seq

Organism

Megalopta genalis

Instrument

Illumina HiSeq 2500

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 2615904000 reported

total reads 13079520 reported

n content pct 0.004 measured

pct q20 bases 97.3 measured

pct q30 bases 95 measured

gc content pct 43.6 measured

mean read length 100 measured

mean base quality 36.6 measured

adapter content pct 0.07 measured

duplication rate pct 54.6 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 91/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 95 measured ×1 100%

mean base quality 36.6 measured ×0.6 100%

adapter content pct 0.07 measured ×0.4 100%

duplication rate pct 54.6 measured ×0.4 45%

QC cost 37 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0