SRR4023226

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

99/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Bulk RNA-seq from Drosophila pseudoobscura on an Illumina HiSeq 2000, producing 30.8 million short reads across 6.2 billion bases with lower Q30 coverage (89.7%) and elevated N-content (0.081%). Despite quality concerns, the GC composition (46.9%) and depth support expression studies when stringent filtering is applied.

Data type / assay

bulk-RNA-seq

Organism

Drosophila pseudoobscura

Instrument

Illumina HiSeq 2000

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 6226844324 reported

total reads 30825962 reported

n content pct 0.081 measured

pct q20 bases 93.4 measured

pct q30 bases 89.7 measured

gc content pct 46.9 measured

mean read length 101 measured

mean base quality 34.7 measured

adapter content pct 0.11 measured

duplication rate pct 27.18 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 99/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 89.7 measured ×1 99%

mean base quality 34.7 measured ×0.6 100%

adapter content pct 0.11 measured ×0.4 100%

duplication rate pct 27.18 measured ×0.4 100%

QC cost 34 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0