SRR4023289

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

71/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Short-read bulk RNA-seq from Drosophila pseudoobscura on an Illumina HiSeq 2000 with 34.0 million reads spanning 6.9 billion bases and moderate Q30 coverage (79.8%). The minimal N-content (0.003%) and moderate GC composition (42.9%) provide substantial depth for expression profiling with quality filtering.

Data type / assay

bulk-RNA-seq

Organism

Drosophila pseudoobscura

Instrument

Illumina HiSeq 2000

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 6874146052 reported

total reads 34030426 reported

n content pct 0.003 measured

pct q20 bases 90.6 measured

pct q30 bases 79.8 measured

gc content pct 42.9 measured

mean read length 101 measured

mean base quality 32.4 measured

adapter content pct 0.54 measured

duplication rate pct 34.18 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 71/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 79.8 measured ×1 49%

mean base quality 32.4 measured ×0.6 73%

adapter content pct 0.54 measured ×0.4 100%

duplication rate pct 34.18 measured ×0.4 91%

QC cost 46 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0