SRR4421334

SRA

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

85/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Human RNA-seq via Illumina HiSeq 2500 with moderate depth (28.7M reads) and variable quality (91.5% Q20), adequate for gene expression analysis but with lower per-base confidence than high-quality datasets. Modest depth limits power for detecting lowly-expressed or condition-specific transcripts.

Data type / assay

bulk-RNA-seq

Organism

Homo sapiens

Instrument

Illumina HiSeq 2500

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 5792640880 reported

total reads 28676440 reported

n content pct 0.007 measured

pct q20 bases 91.5 measured

pct q30 bases 83.1 measured

gc content pct 45.3 measured

mean read length 101 measured

mean base quality 33.8 measured

adapter content pct 0.14 measured

duplication rate pct 18.22 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 85/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 83.1 measured ×1 66%

mean base quality 33.8 measured ×0.6 97%

adapter content pct 0.14 measured ×0.4 100%

duplication rate pct 18.22 measured ×0.4 100%

QC cost 13 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0