SRR446009

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

82/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Large bulk RNA-seq (179M reads); B grade driven by low mean_base_quality=30 and moderate duplication (34.18%), both reducing confidence in low-abundance transcript quantification and isoform discovery at scale.

Data type / assay

bulk-RNA-seq

Organism

Apis mellifera

Instrument

Illumina HiSeq 2000

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 36292149008 reported

total reads 179664104 reported

n content pct 0.037 measured

pct q20 bases 100 measured

pct q30 bases 100 measured

gc content pct 36.7 measured

mean read length 101 measured

mean base quality 30 measured

adapter content pct 0.79 measured

duplication rate pct 34.18 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 82/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 100 measured ×1 100%

mean base quality 30 measured ×0.6 33%

adapter content pct 0.79 measured ×0.4 100%

duplication rate pct 34.18 measured ×0.4 91%

QC cost 18 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0