SRR453174

ENA first seen 2013

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

78/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

RNA-seq with adequate Q30 (100%) but concerning mean base quality of only 30 (scored 33/100), suggesting quality degradation despite apparent pass rates. Duplication at 44.82% also elevated (scored 67/100). Usable but quality is compromised; recommend careful QC during downstream analysis.

Data type / assay

bulk-RNA-seq

Organism

Mus musculus

Instrument

Illumina Genome Analyzer II

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 6299386768 reported

total reads 41443334 reported

n content pct 0.011 measured

pct q20 bases 100 measured

pct q30 bases 100 measured

gc content pct 51.4 measured

mean read length 76 measured

mean base quality 30 measured

adapter content pct 0.01 measured

duplication rate pct 44.82 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 78/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 100 measured ×1 100%

mean base quality 30 measured ×0.6 33%

adapter content pct 0.01 measured ×0.4 100%

duplication rate pct 44.82 measured ×0.4 67%

QC cost 57 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0