SRR4733608

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

75/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

RNA-seq with moderate base quality (Q30=85.6%, scored 78/100) but very high duplication (70.68%, scored 10/100), the dominant negative driver. This combination suggests either PCR over-amplification or genuine biological complexity. Caution recommended for abundance studies; investigate library characteristics before reuse.

Data type / assay

bulk-RNA-seq

Organism

Drosophila pseudoobscura

Instrument

Illumina HiSeq 2000

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 459892592 reported

total reads 2276696 reported

n content pct 0.004 measured

pct q20 bases 94.4 measured

pct q30 bases 85.6 measured

gc content pct 42.9 measured

mean read length 101 measured

mean base quality 33.8 measured

adapter content pct 0.2 measured

duplication rate pct 70.68 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 75/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 85.6 measured ×1 78%

mean base quality 33.8 measured ×0.6 97%

adapter content pct 0.2 measured ×0.4 100%

duplication rate pct 70.68 measured ×0.4 10%

QC cost 55 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0