SRR505574

ENA first seen 2018

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

33/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Aquilegia coerulea 454 amplicon with F-grade quality unsuitable for variant detection: only 61.6% of bases meet Q30 (scored 0/100), making SNP calls unreliable. The older 454 GS FLX platform's lower throughput and quality further compromise utility for amplicon-based genotyping.

Data type / assay

amplicon

Organism

Aquilegia coerulea

Instrument

454 GS FLX

Platform

LS454

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 825386025 reported

total reads 1736095 reported

n content pct 0.056 measured

pct q20 bases 61.6 measured

pct q30 bases 61.6 measured

gc content pct 42.5 measured

mean read length 289.9 measured

mean base quality 19.6 measured

adapter content pct 0 measured

duplication rate pct 19.47 measured

How this grade was computed

Weighted mean of 2 scored metric(s) → 33/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 61.6 measured ×1 0%

adapter content pct 0 measured ×0.5 100%

QC cost 44 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0