SRR5059366

This is a miRNA-Seq small-RNA library from mouse, sequenced on an Illumina HiSeq 1000, and on quality grounds it earns a solid B (83/100): base quality is excellent, with Q30 at 95.4%, a mean base quality of 36.8, and effectively no adapter or N contamination, so the underlying basecalls are trustworthy and the ~22 bp mean read length is exactly what you expect for mature miRNA inserts. The single factor pulling the grade down is the duplication rate of 96.92%, which scored 0 and is the main brake on the score — but note that for small-RNA/miRNA libraries extreme duplication is largely expected, since the true biological diversity of mature miRNAs is small and identical reads accumulate legitimately, so this should be interpreted as low complexity rather than a technical failure. Practically, the data are clean and reusable for miRNA expression quantification, though the very high duplication means you should rely on collapsing identical reads and treat any analysis requiring sequence complexity (e.g. novel-RNA discovery) with caution. One caveat on the rating itself: evidence_strength is reported as 1, and several headline fields (read/base counts, checksum) are reported rather than measured, so while the QC-critical quality and duplication metrics here are genuinely measured, treat the broader characterization as provisional pending a fuller measured pass.