SRR5109819

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

83/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Apis mellifera miRNA-seq with B-grade quality severely compromised by catastrophic duplication of 84.36% (scored 0/100), reflecting extreme PCR amplification bias that will artificially inflate miRNA abundance estimates. Despite excellent base quality (Q30=96.9%), quantitation is unreliable.

Data type / assay

bulk-RNA-seq

Organism

Apis mellifera

Instrument

Illumina HiSeq 2000

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 1202033000 reported

total reads 24040660 reported

n content pct 0.002 measured

pct q20 bases 98.4 measured

pct q30 bases 96.9 measured

gc content pct 54.3 measured

mean read length 50 measured

mean base quality 36.8 measured

adapter content pct 0 measured

duplication rate pct 84.36 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 83/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 96.9 measured ×1 100%

mean base quality 36.8 measured ×0.6 100%

adapter content pct 0 measured ×0.4 100%

duplication rate pct 84.36 measured ×0.4 0%

QC cost 27 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0