SRR513196

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

71/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Bulk RNA-seq from A. gambiae. Grade C (71/100) is driven by severe PCR duplication at 62.13% and borderline mean base quality (30), signaling over-amplification and low input material. Duplication at this level will inflate fold-changes and mask biological replication; expression estimates require aggressive deduplication and careful normalization before reuse.

Data type / assay

bulk-RNA-seq

Organism

Anopheles gambiae

Instrument

Illumina Genome Analyzer IIx

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 262272580 reported

total reads 3450955 reported

n content pct 0.042 measured

pct q20 bases 100 measured

pct q30 bases 100 measured

gc content pct 47.6 measured

mean read length 76 measured

mean base quality 30 measured

adapter content pct 0.1 measured

duplication rate pct 62.13 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 71/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 100 measured ×1 100%

mean base quality 30 measured ×0.6 33%

adapter content pct 0.1 measured ×0.4 100%

duplication rate pct 62.13 measured ×0.4 29%

QC cost 25 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0