SRR5219350

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

92/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Short-read bulk RNA-seq from Anopheles gambiae. Strong QC (A/92) with excellent Q30 (98.2%) and base quality (38.0%), constrained by moderate-to-high duplication at 51.18%. Basecall quality is exceptional; duplication suggests some PCR amplification but remains manageable for most applications.

Data type / assay

bulk-RNA-seq

Organism

Anopheles gambiae

Instrument

Illumina HiSeq 2000

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 567492600 reported

total reads 2837463 reported

n content pct 0.003 measured

pct q20 bases 99.4 measured

pct q30 bases 98.2 measured

gc content pct 35 measured

mean read length 100 measured

mean base quality 38 measured

adapter content pct 0.67 measured

duplication rate pct 51.18 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 92/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 98.2 measured ×1 100%

mean base quality 38 measured ×0.6 100%

adapter content pct 0.67 measured ×0.4 100%

duplication rate pct 51.18 measured ×0.4 53%

QC cost 5 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0