SRR5219352

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

89/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Short-read bulk RNA-seq from Anopheles gambiae. Acceptable QC (B/89) with high Q30 (98.2%) and base quality (38.1), but duplication at 59.33% is notably elevated and is the primary limiting factor. Quality remains good for quantification, though duplication concerns limit sensitive variant calling.

Data type / assay

bulk-RNA-seq

Organism

Anopheles gambiae

Instrument

Illumina HiSeq 2000

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 325055200 reported

total reads 1625276 reported

n content pct 0.002 measured

pct q20 bases 99.4 measured

pct q30 bases 98.2 measured

gc content pct 32.3 measured

mean read length 100 measured

mean base quality 38.1 measured

adapter content pct 0.69 measured

duplication rate pct 59.33 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 89/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 98.2 measured ×1 100%

mean base quality 38.1 measured ×0.6 100%

adapter content pct 0.69 measured ×0.4 100%

duplication rate pct 59.33 measured ×0.4 35%

QC cost 4 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0