Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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SRR5241768

ENA first seen 2018

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

83/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:opus

This is a small-RNA (miRNA-Seq) library from mouse run on an Illumina MiSeq, with short ~36 bp reads consistent with mature microRNA inserts. In QC terms it earns a solid B (83/100): base quality is excellent and trustworthy — pct_q30 of 96.6% and a mean base quality of 36.2 both score full marks, with zero adapter and N content, so the reads themselves are clean and reliable for mapping. The grade is held back almost entirely by a 97.27% duplication rate, which scored 0 and is the single dominant penalty; however, for miRNA-Seq this extreme duplication is largely expected biology rather than a library defect, because the small, highly expressed microRNA repertoire produces many genuinely identical reads, so a generic dedup-based penalty likely overstates the problem here. All contributing metrics were directly measured (evidence_strength is full), so this reading is not provisional — just be aware that for differential-expression reuse you should treat the duplication flag as assay-typical and verify complexity at the miRNA level rather than discarding the dataset on the raw duplication number alone.

Data type / assay
bulk-RNA-seq
Organism
Mus musculus
Instrument
Illumina MiSeq
Platform
ILLUMINA
N numbers (samples, groups)
1 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 208908936 reported
total reads 5803026 reported
n content pct 0 measured
pct q20 bases 97.6 measured
pct q30 bases 96.6 measured
gc content pct 50.1 measured
mean read length 36 measured
mean base quality 36.2 measured
adapter content pct 0 measured
duplication rate pct 97.27 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 83/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 96.6 measured ×1 100%
mean base quality 36.2 measured ×0.6 100%
adapter content pct 0 measured ×0.4 100%
duplication rate pct 97.27 measured ×0.4 0%
QC cost 17 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0

Scientific quality

Based on hands-on reproduction of the papers that use this dataset. A reproducible paper that stands on this data is positive evidence; a flagged one is a prompt to look closer — never a verdict on the dataset itself without the evidence.

1 studies use it 1 reproduced mean score 89