SRR5260368

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

99/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

High-quality bulk-RNA-seq from Nilaparvata lugens with outstanding base quality (Q30=98.2%, highest mean quality 38.8) and modest duplication (32.1%) across 20M reads of short length (51bp). Exceptional quality metrics support sensitive isoform and variant detection despite the short read length.

Data type / assay

bulk-RNA-seq

Organism

Nilaparvata lugens

Instrument

Illumina HiSeq 2500

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 1024060059 reported

total reads 20079609 reported

n content pct 0.006 measured

pct q20 bases 99.3 measured

pct q30 bases 98.2 measured

gc content pct 44.8 measured

mean read length 51 measured

mean base quality 38.8 measured

adapter content pct 0.03 measured

duplication rate pct 32.05 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 99/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 98.2 measured ×1 100%

mean base quality 38.8 measured ×0.6 100%

adapter content pct 0.03 measured ×0.4 100%

duplication rate pct 32.05 measured ×0.4 95%

QC cost 26 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0