SRR5309767

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

100/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Temnothorax longispinosus bulk RNA-seq achieving perfect QC (100/100, grade A). Very low duplication (12.13%) and solid base quality (91% Q30) provide excellent margin for robust differential expression analysis without bias correction.

Data type / assay

bulk-RNA-seq

Organism

Temnothorax longispinosus

Instrument

Illumina HiSeq 2000

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 808000000 reported

total reads 4000000 reported

n content pct 0.007 measured

pct q20 bases 97 measured

pct q30 bases 91 measured

gc content pct 43.8 measured

mean read length 101 measured

mean base quality 35.4 measured

adapter content pct 0.35 measured

duplication rate pct 12.13 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 100/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 91 measured ×1 100%

mean base quality 35.4 measured ×0.6 100%

adapter content pct 0.35 measured ×0.4 100%

duplication rate pct 12.13 measured ×0.4 100%

QC cost 17 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0