SRR5422766

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

21/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Short-read bulk RNA-seq from Aedes albopictus. Fails QC (F/21) due to critically low sequence quality: only 65.4% of bases reach Q30 (typical threshold ≥90%), which will compromise variant calling and expression quantification. Paired with high duplication (63.34%), this data is unsuitable for reuse without extensive remediation.

Data type / assay

bulk-RNA-seq

Organism

Aedes albopictus

Instrument

Illumina MiSeq

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 279109386 reported

total reads 560920 reported

n content pct 5.174 measured

pct q20 bases 76.4 measured

pct q30 bases 65.4 measured

gc content pct 47.7 measured

mean read length 263.7 measured

mean base quality 27.9 measured

adapter content pct 1.63 measured

duplication rate pct 63.34 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 21/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 65.4 measured ×1 0%

mean base quality 27.9 measured ×0.6 0%

adapter content pct 1.63 measured ×0.4 100%

duplication rate pct 63.34 measured ×0.4 26%

QC cost 4 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0