SRR5422780

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

20/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Short-read bulk RNA-seq from Aedes albopictus. Failed QC (F/20) with poor Q30 (68%) and the worst duplication rate in this cohort (78.64%), indicating sequencing failure or severe over-clustering. This dataset is unsuitable for any analysis and should be discarded.

Data type / assay

bulk-RNA-seq

Organism

Aedes albopictus

Instrument

Illumina MiSeq

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 323556619 reported

total reads 670001 reported

n content pct 2.847 measured

pct q20 bases 79.1 measured

pct q30 bases 68 measured

gc content pct 49 measured

mean read length 266 measured

mean base quality 28.8 measured

adapter content pct 0.98 measured

duplication rate pct 78.64 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 20/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 68 measured ×1 0%

mean base quality 28.8 measured ×0.6 13%

adapter content pct 0.98 measured ×0.4 100%

duplication rate pct 78.64 measured ×0.4 0%

QC cost 4 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0