SRR5422781

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

18/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Short-read bulk RNA-seq from Aedes albopictus. Failed QC (F/18) with Q30 capped at 67.2% and extraordinarily high duplication at 73.44%—the second-worst in this cohort. With quality metrics well below usable thresholds, this sequencing run should be repeated before inclusion in any analysis.

Data type / assay

bulk-RNA-seq

Organism

Aedes albopictus

Instrument

Illumina MiSeq

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 423938835 reported

total reads 860297 reported

n content pct 4.944 measured

pct q20 bases 77.8 measured

pct q30 bases 67.2 measured

gc content pct 47.3 measured

mean read length 265.3 measured

mean base quality 28.4 measured

adapter content pct 2.45 measured

duplication rate pct 73.44 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 18/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 67.2 measured ×1 0%

mean base quality 28.4 measured ×0.6 7%

adapter content pct 2.45 measured ×0.4 98%

duplication rate pct 73.44 measured ×0.4 4%

QC cost 7 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0