SRR5461073

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

22/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Small-RNA sequencing. Grade F (22/100) fails on base quality: 57% Q30 and 24.1 mean Phred both scored 0/100, making reliable alignment and expression estimates unreliable. Reuse not recommended without complementary validation data.

Data type / assay

bulk-RNA-seq

Organism

Drosophila ananassae

Instrument

Illumina Genome Analyzer II

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 1473578460 reported

total reads 40932735 reported

n content pct 1.35 measured

pct q20 bases 68.3 measured

pct q30 bases 57 measured

gc content pct 50.4 measured

mean read length 36 measured

mean base quality 24.1 measured

adapter content pct 0 measured

duplication rate pct 60.84 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 22/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 57 measured ×1 0%

mean base quality 24.1 measured ×0.6 0%

adapter content pct 0 measured ×0.4 100%

duplication rate pct 60.84 measured ×0.4 32%

QC cost 28 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0