SRR5641349

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

93/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Silkworm transcriptome from HiSeq 2500 with 25.7 million short reads and outstanding quality (98.8% ≥Q20, 96.6% ≥Q30). The consistent excellent accuracy across replicates supports robust isoform quantification and differential expression analysis.

Data type / assay

bulk-RNA-seq

Organism

Bombyx mori

Instrument

Illumina HiSeq 2500

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 7696817400 reported

total reads 25656058 reported

n content pct 0 measured

pct q20 bases 98.8 measured

pct q30 bases 96.6 measured

gc content pct 48 measured

mean read length 150 measured

mean base quality 40.3 measured

adapter content pct 1.32 measured

duplication rate pct 47.87 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 93/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 96.6 measured ×1 100%

mean base quality 40.3 measured ×0.6 100%

adapter content pct 1.32 measured ×0.4 100%

duplication rate pct 47.87 measured ×0.4 60%

QC cost 38 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0