Corpus 1,285 assessed · 1,186 scored · 647 reproduced ≥75 · 174 flagged ·∅ 73.9/100
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SRR5980085

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

96/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Bulk RNA-seq from alfalfa leafcutter bee (Megachile rotundata) on HiSeq 2000 with 4.6M reads and solid 97.8% Q20, 92.9% Q30 supporting gene expression profiling of solitary bee pollinators. The 935M bases and 40.5% GC enable comparative studies between solitary and social hymenopterans and annotation of bee-specific transcripts. Reusable for investigating foraging ecology, nest-building behavior, and reproductive physiology distinct from social bee models.

Data type / assay
bulk-RNA-seq
Organism
Megachile rotundata
Instrument
Illumina HiSeq 2000
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
1 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 935990836 reported
total reads 4633618 reported
n content pct 0.002 measured
pct q20 bases 97.8 measured
pct q30 bases 92.9 measured
gc content pct 40.5 measured
mean read length 101 measured
mean base quality 36.2 measured
adapter content pct 3.74 measured
duplication rate pct 35.14 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 96/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 92.9 measured ×1 100%
mean base quality 36.2 measured ×0.6 100%
adapter content pct 3.74 measured ×0.4 90%
duplication rate pct 35.14 measured ×0.4 89%
QC cost 50 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0