SRR7426848

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

74/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

This Anopheles gambiae (malaria mosquito) HiSeq 3000 library shows elevated N-content (9.09%) and lower Q20 (87.7%) despite high depth, suggesting potential sequencing artifacts or sample contamination. Use with caution for SNP calling or expression studies; technical QC should precede downstream analysis.

Data type / assay

bulk-RNA-seq

Organism

Anopheles gambiae

Instrument

Illumina HiSeq 3000

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 3369673186 reported

total reads 14705886 reported

n content pct 9.09 measured

pct q20 bases 87.7 measured

pct q30 bases 83.3 measured

gc content pct 43.8 measured

mean read length 111.1 measured

mean base quality 34.9 measured

adapter content pct 0.02 measured

duplication rate pct 61.4 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 74/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 83.3 measured ×1 67%

mean base quality 34.9 measured ×0.6 100%

adapter content pct 0.02 measured ×0.4 100%

duplication rate pct 61.4 measured ×0.4 30%

QC cost 35 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0