SRR8587237

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

60/100 · D

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Bulk RNA-seq (NextSeq 500, Musca domestica). Grade D—avoid if alternatives exist. Extreme duplication (72.54%, scored 6/100), Q30 only 82.6%, and mean base quality 32 combine to create a heavily compromised dataset for both detection and quantification.

Data type / assay

bulk-RNA-seq

Organism

Musca domestica

Instrument

NextSeq 500

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 3366973141 reported

total reads 44702325 reported

n content pct 0.013 measured

pct q20 bases 87.1 measured

pct q30 bases 82.6 measured

gc content pct 32.1 measured

mean read length 75.3 measured

mean base quality 32 measured

adapter content pct 0.01 measured

duplication rate pct 72.54 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 60/100

The D grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 82.6 measured ×1 63%

mean base quality 32 measured ×0.6 67%

adapter content pct 0.01 measured ×0.4 100%

duplication rate pct 72.54 measured ×0.4 6%

QC cost 51 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0