SRR8587238

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

57/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Bulk-RNA-seq from Musca domestica (NextSeq 500), grade F—NOT recommended for reuse. Catastrophic duplication (76.85%, highest in cohort) combined with subpar Q30 (81.7%) and the lowest base quality (31.8%) severely compromise reliability; expression estimates and variant calls will be heavily confounded by PCR bias. Reuse would require extraordinary justification.

Data type / assay

bulk-RNA-seq

Organism

Musca domestica

Instrument

NextSeq 500

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 1292611841 reported

total reads 17154576 reported

n content pct 0.003 measured

pct q20 bases 86.8 measured

pct q30 bases 81.7 measured

gc content pct 28.2 measured

mean read length 75.4 measured

mean base quality 31.8 measured

adapter content pct 0 measured

duplication rate pct 76.85 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 57/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 81.7 measured ×1 59%

mean base quality 31.8 measured ×0.6 63%

adapter content pct 0 measured ×0.4 100%

duplication rate pct 76.85 measured ×0.4 0%

QC cost 54 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0