SRR8606537

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

83/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Short-read RNA-seq with exceptional base quality (Q30 99.6%, mean 40.8) but severely hampered by extremely high duplication (81.37%), which inflates variance estimates and depletes effective library diversity. Very short reads (24.6 bp mean) further limit isoform discrimination; Grade B is generous—deep resequencing recommended.

Data type / assay

bulk-RNA-seq

Organism

Aedes aegypti

Instrument

Illumina HiSeq 2500

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 361985115 reported

total reads 14706143 reported

n content pct 0.014 measured

pct q20 bases 99.9 measured

pct q30 bases 99.6 measured

gc content pct 45.6 measured

mean read length 24.6 measured

mean base quality 40.8 measured

adapter content pct 0 measured

duplication rate pct 81.37 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 83/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 99.6 measured ×1 100%

mean base quality 40.8 measured ×0.6 100%

adapter content pct 0 measured ×0.4 100%

duplication rate pct 81.37 measured ×0.4 0%

QC cost 40 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0