SRR8754039

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

86/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Bulk-RNA-seq from Apis mellifera (NextSeq 500), grade B. Duplication at 68.28% (second-highest in cohort) heavily penalizes reusability despite acceptable Q30 (92.1%) and base quality (36.1); NextSeq platform combined with high duplication suggests library complexity issues that will distort transcript quantification. Reuse requires intensive deduplication.

Data type / assay

bulk-RNA-seq

Organism

Apis mellifera

Instrument

NextSeq 500

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 2908985400 reported

total reads 14544927 reported

n content pct 0.001 measured

pct q20 bases 97.5 measured

pct q30 bases 92.1 measured

gc content pct 39.1 measured

mean read length 100 measured

mean base quality 36.1 measured

adapter content pct 0.51 measured

duplication rate pct 68.28 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 86/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 92.1 measured ×1 100%

mean base quality 36.1 measured ×0.6 100%

adapter content pct 0.51 measured ×0.4 100%

duplication rate pct 68.28 measured ×0.4 15%

QC cost 53 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0