SRR8884714

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

96/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Myzus persicae RNA-seq grading A (96/100), anchored by excellent base quality (Q30=95.1%, mean=35.7) but modestly penalized by 6% adapter content (77/100). Longer 125bp reads and manageable 30% duplication support transcript quantification well; minor adapter levels are routine and pose minimal mapping bias.

Data type / assay

bulk-RNA-seq

Organism

Myzus persicae

Instrument

Illumina HiSeq 2000

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 7970604000 reported

total reads 31882416 reported

n content pct 0.008 measured

pct q20 bases 97.4 measured

pct q30 bases 95.1 measured

gc content pct 42.6 measured

mean read length 125 measured

mean base quality 35.7 measured

adapter content pct 6.06 measured

duplication rate pct 29.9 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 96/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 95.1 measured ×1 100%

mean base quality 35.7 measured ×0.6 100%

adapter content pct 6.06 measured ×0.4 77%

duplication rate pct 29.9 measured ×0.4 100%

QC cost 19 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0