Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
← Dataset search

SRR900572

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

85/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Butterfly bulk RNA-seq with moderate quality but severe complexity issues. Grade B (85/100) masks a critical problem: 71.15% duplication rate (scored only 9/100) indicates technical replicates or low-complexity library, which will bias transcript counts and inflate false positives in differential expression. Duplication this high matters for quantitative accuracy—proceed with duplicate-aware tools or deep sequencing pooling.

Data type / assay
bulk-RNA-seq
Organism
Melitaea cinxia
Instrument
Illumina HiSeq 2000
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
1 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 850231938 reported
total reads 4209069 reported
n content pct 0.007 measured
pct q20 bases 95.2 measured
pct q30 bases 90 measured
gc content pct 27.7 measured
mean read length 101 measured
mean base quality 35.1 measured
adapter content pct 0.35 measured
duplication rate pct 71.15 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 85/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 90 measured ×1 100%
mean base quality 35.1 measured ×0.6 100%
adapter content pct 0.35 measured ×0.4 100%
duplication rate pct 71.15 measured ×0.4 9%
QC cost 29 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0