SRR900579

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

86/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Bulk-RNA-seq from Melitaea cinxia (HiSeq 2000), grade B. Duplication (62.45%) and Q30 (88.9%, lowest in dataset) are both marginal; the combination suggests lower sequencing depth or elevated PCR cycles that reduce per-base accuracy. Suitable for reuse in robust analyses but not for sensitive applications.

Data type / assay

bulk-RNA-seq

Organism

Melitaea cinxia

Instrument

Illumina HiSeq 2000

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 755884202 reported

total reads 3742001 reported

n content pct 0.001 measured

pct q20 bases 95.8 measured

pct q30 bases 88.9 measured

gc content pct 31.1 measured

mean read length 101 measured

mean base quality 35 measured

adapter content pct 0.13 measured

duplication rate pct 62.45 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 86/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 88.9 measured ×1 95%

mean base quality 35 measured ×0.6 100%

adapter content pct 0.13 measured ×0.4 100%

duplication rate pct 62.45 measured ×0.4 28%

QC cost 13 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0