SRR900651

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

85/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Bulk RNA-seq (HiSeq 2000, Melitaea cinxia). Grade B, severely limited by very high duplication at 65.7% (scored 21/100). Acceptable Q30 (89.3%) but duplication bias is severe—consider spike-in or RT-qPCR validation for key expression claims.

Data type / assay

bulk-RNA-seq

Organism

Melitaea cinxia

Instrument

Illumina HiSeq 2000

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 1004973432 reported

total reads 4975116 reported

n content pct 0.001 measured

pct q20 bases 95.8 measured

pct q30 bases 89.3 measured

gc content pct 31 measured

mean read length 101 measured

mean base quality 35.1 measured

adapter content pct 0.27 measured

duplication rate pct 65.7 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 85/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 89.3 measured ×1 97%

mean base quality 35.1 measured ×0.6 100%

adapter content pct 0.27 measured ×0.4 100%

duplication rate pct 65.7 measured ×0.4 21%

QC cost 8 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0