Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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SRR9041549

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

41/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Honey bee bulk RNA-seq with poor technical performance. Grade F (41/100) shows multiple failures: Q30 only 75.2% (scored 26/100), mean quality 30.7 (scored 45/100), and duplication 67.63% (scored 16/100). The combination of low per-base quality and high duplication makes this unsuitable for most downstream analyses—expression calls will have high error rates and false duplicate-driven signals. Not recommended for reuse.

Data type / assay
bulk-RNA-seq
Organism
Apis mellifera
Instrument
NextSeq 500
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
1 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 10731288222 reported
total reads 43892205 reported
n content pct 0.051 measured
pct q20 bases 83.8 measured
pct q30 bases 75.2 measured
gc content pct 49 measured
mean read length 124.8 measured
mean base quality 30.7 measured
adapter content pct 0.51 measured
duplication rate pct 67.63 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 41/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 75.2 measured ×1 26%
mean base quality 30.7 measured ×0.6 45%
adapter content pct 0.51 measured ×0.4 100%
duplication rate pct 67.63 measured ×0.4 16%
QC cost 8 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0