SRR935072

ENA first seen 2014

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

74/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

This Buchnera aphidicola str. Ua bulk RNA-Seq on HiSeq 2000 spans 1.2 Gb with 34.8 million reads and good quality (94.8% Q20, 85.5% Q30), providing transcriptome data for this obligate aphid symbiont. The dataset enables studying symbiotic-gene expression. Symbiotic microbiology and aphid-host interaction studies can use this bacterial RNA-seq.

Data type / assay

bulk-RNA-seq

Organism

Buchnera aphidicola str. Ua (Uroleucon ambrosiae)

Instrument

Illumina HiSeq 2000

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 1218611380 reported

total reads 34817468 reported

n content pct 0.002 measured

pct q20 bases 94.8 measured

pct q30 bases 85.5 measured

gc content pct 48.5 measured

mean read length 35 measured

mean base quality 34.7 measured

adapter content pct 0 measured

duplication rate pct 89.38 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 74/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 85.5 measured ×1 78%

mean base quality 34.7 measured ×0.6 100%

adapter content pct 0 measured ×0.4 100%

duplication rate pct 89.38 measured ×0.4 0%

QC cost 16 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0