SRR9831073

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

98/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Bulk RNA-seq of Myzus persicae from HiSeq 2500, 36M reads, 7.2B bases, 100bp. Excellent A grade (98/100, all measured) with Q30 of 94.7%, base quality of 36.5, and minimal adapter content; duplication of 34.48% (scored 90) is the only minor limiting factor but acceptable. Highly suitable for differential expression and assembly.

Data type / assay

bulk-RNA-seq

Organism

Myzus persicae

Instrument

Illumina HiSeq 2500

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 7219899200 reported

total reads 36099496 reported

n content pct 0.009 measured

pct q20 bases 97.4 measured

pct q30 bases 94.7 measured

gc content pct 40.4 measured

mean read length 100 measured

mean base quality 36.5 measured

adapter content pct 1.41 measured

duplication rate pct 34.48 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 98/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 94.7 measured ×1 100%

mean base quality 36.5 measured ×0.6 100%

adapter content pct 1.41 measured ×0.4 100%

duplication rate pct 34.48 measured ×0.4 90%

QC cost 31 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0