SRX020786

ENA first seen 2013

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

67/100 · D

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Bulk RNA-seq of Homo sapiens from Genome Analyzer, 9.7M reads, 310M bases, 32bp. Grade D (67/100) with severe duplication of 70.49% (scored 10/100), indicating over-amplification and PCR artifacts that corrupt abundance estimates; Q30 of 81.3% is secondary concern. Duplication this high compromises differential expression analysis.

Data type / assay

bulk-RNA-seq

Organism

Homo sapiens

Instrument

Illumina Genome Analyzer

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

2 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 310072576 reported

total reads 9689768 reported

n content pct 0.002 measured

pct q20 bases 87.7 measured

pct q30 bases 81.3 measured

gc content pct 49.4 measured

mean read length 32 measured

mean base quality 35.1 measured

adapter content pct 0 measured

duplication rate pct 70.49 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 67/100

The D grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 81.3 measured ×1 56%

mean base quality 35.1 measured ×0.6 100%

adapter content pct 0 measured ×0.4 100%

duplication rate pct 70.49 measured ×0.4 10%

QC cost 29 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0