SRX021650

ENA first seen 2017

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

70/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

EST/454 sequencing of Zostera marina, 867k long reads (418 bp mean). Grade C (70/100) driven by Q30 of 75.9% (measured, scored 30/100)—below premium standards but acceptable for EST assembly. Mean base quality of 33.9 is respectable; the 454 platform's inherent limitations and 17.6% duplication are typical for this assay type.

Data type / assay

bulk-RNA-seq

Organism

Zostera marina

Instrument

454 GS FLX

Platform

LS454

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 451045207 reported

total reads 866838 reported

n content pct 0.048 measured

pct q20 bases 90.3 measured

pct q30 bases 75.9 measured

gc content pct 43.4 measured

mean read length 418.3 measured

mean base quality 33.9 measured

adapter content pct 0 measured

duplication rate pct 17.61 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 70/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 75.9 measured ×1 30%

mean base quality 33.9 measured ×0.6 98%

adapter content pct 0 measured ×0.4 100%

duplication rate pct 17.61 measured ×0.4 100%

QC cost 18 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0