SRX032867

SRA first seen 2016

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

60/100 · D

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

EST/454 Titanium of Ceratodon purpureus, 1.25M long reads (383 bp mean). Grade D (60/100) with measured Q30 of 72.2% (scored 11/100) being the limiting factor—below acceptable thresholds for downstream processing. The 454 platform's higher error rate and 12.4% duplication further limit utility; measured base quality of 33.2 provides limited compensation.

Data type / assay

bulk-RNA-seq

Organism

Ceratodon purpureus

Instrument

454 GS FLX Titanium

Platform

LS454

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 633164479 reported

total reads 1252018 reported

n content pct 0.022 measured

pct q20 bases 90.7 measured

pct q30 bases 72.2 measured

gc content pct 52.4 measured

mean read length 383.8 measured

mean base quality 33.2 measured

adapter content pct 0 measured

duplication rate pct 12.42 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 60/100

The D grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 72.2 measured ×1 11%

mean base quality 33.2 measured ×0.6 87%

adapter content pct 0 measured ×0.4 100%

duplication rate pct 12.42 measured ×0.4 100%

QC cost 30 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0