Corpus 1,284 assessed · 1,185 scored · 647 reproduced ≥75 · 173 flagged ·∅ 73.9/100
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SRX039631

ENA first seen 2012

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

71/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

This Abies balsamea (balsam fir) bulk RNA-Seq dataset from the 454 GS FLX Titanium platform generated 797,060 long reads (435.3 million bases) with good quality (90.9% Q20, 76% Q30 bases) and minimal N-content (0.023%). The long-read EST approach enables full-length transcript recovery in this conifer species. Search terms: plant transcriptomics, 454 long-read sequencing, conifer genomics, full-length cDNA, all relevant to discovering novel isoforms in forest tree transcriptomes.

Data type / assay
bulk-RNA-seq
Organism
Abies balsamea
Instrument
454 GS FLX Titanium
Platform
LS454
Files available
FASTQ (raw reads)
N numbers (samples, groups)
1 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 435251705 reported
total reads 797060 reported
n content pct 0.023 measured
pct q20 bases 90.9 measured
pct q30 bases 76 measured
gc content pct 46.1 measured
mean read length 417.2 measured
mean base quality 34.1 measured
adapter content pct 0 measured
duplication rate pct 22.58 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 71/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 76 measured ×1 30%
mean base quality 34.1 measured ×0.6 100%
adapter content pct 0 measured ×0.4 100%
duplication rate pct 22.58 measured ×0.4 100%
QC cost 36 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0