SRX106024

ENA first seen 2014

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

67/100 · D

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Bulk RNA-seq of Drosophila miranda from Genome Analyzer II, 27M reads, 5.5B bases. Grade D (67/100) due to catastrophic adapter content of 21.35% (scored 0/100), suggesting significant primer/linker carryover; mean base quality of 30 (scored 33/100) compounds the issue. Adapter trimming is mandatory and may still not fully rescue transcript mapping.

Data type / assay

bulk-RNA-seq

Organism

Drosophila miranda

Instrument

Illumina Genome Analyzer II

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 5509036516 reported

total reads 27272458 reported

n content pct 0.02 measured

pct q20 bases 100 measured

pct q30 bases 100 measured

gc content pct 51.5 measured

mean read length 101 measured

mean base quality 30 measured

adapter content pct 21.35 measured

duplication rate pct 19.07 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 67/100

The D grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 100 measured ×1 100%

mean base quality 30 measured ×0.6 33%

adapter content pct 21.35 measured ×0.4 0%

duplication rate pct 19.07 measured ×0.4 100%

QC cost 21 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0