SRX1176317

ENA first seen 2015

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

73/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

This Illumina HiSeq 2000 miRNA-Seq of Hordeum vulgare (barley) generates ~18 million reads (~388 Mb) with good quality (93.1% Q20, 84.8% Q30), enabling plant small-RNA profiling relevant to grain-crop biology. The dataset supports plant systems biology. Researchers seeking plant miRNA-seq will find this useful.

Data type / assay

bulk-RNA-seq

Organism

Hordeum vulgare subsp. vulgare

Instrument

Illumina HiSeq 2000

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 388327433 reported

total reads 18746449 reported

n content pct 0.009 measured

pct q20 bases 93.1 measured

pct q30 bases 84.8 measured

gc content pct 50.4 measured

mean read length 20.8 measured

mean base quality 33.5 measured

adapter content pct 0 measured

duplication rate pct 69.27 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 73/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 84.8 measured ×1 74%

mean base quality 33.5 measured ×0.6 92%

adapter content pct 0 measured ×0.4 100%

duplication rate pct 69.27 measured ×0.4 13%

QC cost 34 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0