Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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SRX146601

ENA first seen 2015

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

67/100 · D

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Bulk RNA-Seq of Gasterosteus aculeatus (three-spined stickleback) on Illumina HiSeq 2000 with 32.7 million paired-end reads and consistent quality (88.3% Q20 and Q30) profiles transcriptome variation in this model fish for evolutionary studies. The dataset supports investigations of gene-expression changes underlying adaptive evolution and ecological divergence in stickleback populations.

Data type / assay
bulk-RNA-seq
Organism
Gasterosteus aculeatus
Instrument
Illumina HiSeq 2000
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
2 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 6599322224 reported
total reads 32669912 reported
n content pct 0.007 measured
pct q20 bases 88.3 measured
pct q30 bases 88.3 measured
gc content pct 47.6 measured
mean read length 101 measured
mean base quality 26.8 measured
adapter content pct 0.24 measured
duplication rate pct 41.35 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 67/100

The D grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 88.3 measured ×1 92%
mean base quality 26.8 measured ×0.6 0%
adapter content pct 0.24 measured ×0.4 100%
duplication rate pct 41.35 measured ×0.4 75%
QC cost 32 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0