SRX152529

ENA first seen 2015

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

66/100 · D

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

This Illumina HiSeq 2000 bulk RNA-seq of Protopterus annectens (lungfish) generates ~25 million reads (~3.8 Gb) with moderate quality (90% Q20/Q30), enabling transcriptome profiling of this ancient fish species. The dataset supports evolutionary-genomics and comparative-biology research. Researchers seeking fish transcriptomics or evolutionary-model organisms will find this useful.

Data type / assay

bulk-RNA-seq

Organism

Protopterus annectens

Instrument

Illumina HiSeq 2000

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

2 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 3864055688 reported

total reads 25421419 reported

n content pct 0.013 measured

pct q20 bases 90 measured

pct q30 bases 90 measured

gc content pct 45.5 measured

mean read length 76 measured

mean base quality 27.3 measured

adapter content pct 0.01 measured

duplication rate pct 55.47 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 66/100

The D grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 90 measured ×1 100%

mean base quality 27.3 measured ×0.6 0%

adapter content pct 0.01 measured ×0.4 100%

duplication rate pct 55.47 measured ×0.4 43%

QC cost 26 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0